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Introduction to the Spin Sequence Comparison Plot

All of the spin comparison functions display their results as points or lines in a two-dimensional plot called a "Spin Sequence Comparison Plot" ( see section SPIN Sequence Comparison Plot). Sets of matches from a single invocation of a comparison command are termed "a result". Each result is plotted using a single colour which can be configured via the results manager ( see section Result manager).

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The diagram above shows the results of a "Find similar spans" search (olive) ( see section Finding Similar Spans), and a "Find matching words" (red) ( see section Finding Matching Words).

At the right hand side is a set of square boxes with the same colours as the dots drawn in the adjacent plot. These icon-like objects represent individual results and allow the user to operate on them. For example clicking with the right mouse button brings up the pop-up menu beneath the "matching words" result contains the results menu for this result ( see section Result manager). These icons can also be used to drag and drop the results to which they correspond. This is activated by pressing the middle mouse button, or Alt left mouse button, over the box and then moving the cursor over the Spin Sequence Comparison Plot to the new location or anywhere outside the Spin Sequence Comparison Plot ( see section Drag and drop).

Crosshairs can be turned on or off using the check button labelled "crosshairs". The x and y positions of the crosshairs are indicated in the two boxes to the right of the check box.

Each sequence displayed in a Spin Sequence Comparison Plot will have a cursor of a particular colour. In the picture above, the sequence on the horizontal axis has a vertical blue cursor whereas the sequence on the vertical axis has a horizontal olive green cursor. In general, the same sequence displayed in several Spin Sequence Comparison Plots will have a cursor of the same colour. The user can move a cursor by clicking and dragging with the middle mouse button, or with Alt left mouse button. This will move all other cursors displayed that relate to the sequence, whether they are in different Spin Sequence Comparison Plots or within the sequence display.

Plots can be enlarged either by resizing the window or zooming. Zooming is achieved by holding down the control key and right mouse button and dragging out a rectangle. This process can be repeated. The Back button will restore the plot to the previous magnification.

To illustrate further uses of the program we include some more screen dumps below.

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The picture above shows the results after performing a "Find similar spans" comparison between the three reading frames of two DNA sequences, producing nine superimposed sets of results.

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Local alignment searches join similar segments with lines. The above screen dump shows such an analysis in which genomic DNA containing 7 exons and is compared to its corresponding cDNA.

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The above screendump shows a global alignment of the same pair of sequences.


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This page is maintained by staden-package. Last generated on 22 October 2002.
URL: http://www.mrc-lmb.cam.ac.uk/pubseq/manual/spin_unix_7.html