Date: Fri May 9 10:42:15 2003 BST, Database BRCA1.0, Contig HSLBRCA1

Summary Mutation Report

Sorted by Position

Name Mutation Effect Fwd Rev Fwd range Rev range
000256_11cF 35512T>C silent L y y 0 - 0 0 - 0
000465_11cF 35512T>V expressed L>[X y y 0 - 0 0 - 0
000437_11dF 36314A>R expressed E>[EG] y y 0 - 0 0 - 0

Sorted by Name

Name Mutation Effect Fwd Rev Fwd range Rev range
Name Mutation Effect Fwd Rev Fwd range Rev range
000256_11cF 35512T>C silent L y y 0 - 0 0 - 0
000437_11dF 36314A>R expressed E>[EG] y y 0 - 0 0 - 0
000465_11cF 35512T>V expressed L>[X y y 0 - 0 0 - 0

Detailed Mutation Report

Sample 000256_11cF, position 35512

MUTA + 35512(253)
T->C Sensitivity= 6.90, Alignment=0.00, Width=0.92, Amplitude=1634 		MUTA - 35512(355)
T->C Sensitivity= 8.13, Alignment=0.07, Width=1.00, Amplitude=1105
seq56_35512_0 trace
000256_11cF.ztr
 seq56_35512_4 trace
000256_11cR.ztr
AGAGAGTAGCAGTATTTCACTGGTACCTGGTACTGATTA AGAGAGTAGCAGTATTTCACTGGTACCTGGTACTGATTA
seq56_35512_1 trace
000906_11cF.ztr (-ve control)
 seq56_35512_5 trace
000906_11cR.ztr (-ve control)
agagagtagcagtatttcattggtacctggtactgatta
seq56_35512_2 trace
(000256_11cF.ztr - 000906_11cF.ztr)
 seq56_35512_6 trace
(000256_11cR.ztr - 000906_11cR.ztr)

Sample 000465_11cF, position 35512

HETE + 35512(252)
CT Ratio=0.68, Alignment=0.08, Amplitude1=0.34, Amplitude2=0.23 		HETE - 35512(355)
CT Ratio=0.78, Alignment=0.08, Amplitude1=0.23, Amplitude2=0.18
seq59_35512_0 trace
000465_11cF.ztr
 seq59_35512_4 trace
000465_11cR.ztr
AGAGAGTAGCAGTATTTCA-TGGTACCTGGTACTGATTA AGAGAGTAGCAGTATTTCATTGGTACCTGGTACTGATTA
seq59_35512_1 trace
000906_11cF.ztr (-ve control)
 seq59_35512_5 trace
000906_11cR.ztr (-ve control)
agagagtagcagtatttcattggtacctggtactgatta
seq59_35512_2 trace
(000465_11cF.ztr - 000906_11cF.ztr)
 seq59_35512_6 trace
(000465_11cR.ztr - 000906_11cR.ztr)

Sample 000437_11dF, position 36314

HETE + 36314(493)
AG Ratio=0.95, Alignment=0.09, Amplitude1=0.27, Amplitude2=0.26 		HETE - 36314(341)
AG Ratio=0.84, Alignment=0.08, Amplitude1=0.19, Amplitude2=0.16
seq66_36314_0 trace
000437_11dF.ztr
 seq66_36314_4 trace
000437_11dR.ztr
AGAGAAAATGTTTTTAAAG-AGCCAGCTCAAGCAATATT AGAGAAAATGTTTTTAAAG-AGCCAGCTCAAGCAATATT
seq66_36314_1 trace
000906_11dF.ztr (-ve control)
 seq66_36314_5 trace
000906_11dR.ztr (-ve control)
agagaaaatgtttttaaagaagccagctcaagcaatatt
seq66_36314_2 trace
(000437_11dF.ztr - 000906_11dF.ztr)
 seq66_36314_6 trace
(000437_11dR.ztr - 000906_11dR.ztr)