Read1 ACCCAG Read2 ACCCAG Read3 ACCCAG Read4 ACC*AG Read5 ACCCAG Consensus ACCCAG
In the above case we see that Read4
has a C
missing. Once again
we must check the traces to be sure that we wish to edit the reading. If so,
then we can either make an edit specifically to Read4
itself (in
"replace" mode) or type C
in the consensus at this column. The latter
will change either the base type of the *
in Read4
to c
,
or will change its confidence value to 0. This depends upon the value of the
"Edit by base type"/"Edit by confidence" setting in the Edit Modes menu.
When replacing base types, it is preferable to use lowercase letters. This makes the modified base stand out. However even when using uppercase letters it is always possible to search for edits at a later stage, although they won't be as obvious to the human eye. Finally, note that the "Allow replace in cons" mode must be set to to enable this solution.