Contigs are joined interactively using the Join Editor. This is simply a pair of contig editor displays stacked above one another with a "differences" line in between. Note that it is essential to align the contigs over the full length of their overlap. It is much more difficult to achieve this after a join has been made, and until the alignment is correct, the consensus sequence will be nonsense.
The few differences between the Join Editor and the Contig Editor can be seen in the figure below. Otherwise all the commands and operations are the same as those for the Contig Editor
One difference is the Lock button. When set (as it is in the
illustration) scrolling either contig, by using the scrollbar or the
four movement buttons, will also scroll the other contig.
The Align button aligns the overlapping consensus sequences and adds pads
The alignment routine assumes that the two contigs are already
in approximately the right relative position (as they are immediately
after the
Join Editor has been invoked from Find Internal Joins, or Find
Repeats). If they are not they must be positioned manually before using
the Align button.
It should be noted
that each of the pair of editors comprising the Contig Editor
maintains its own undo history, and using Align
is likely to add to both undo histories.
Hence, to undo the results of the Align command
the Undo button in both editors must be used.
Note also that storing the undo information takes up a great deal of computer
memory and slows down the alignment process. For this reason the settings menu
contains an option to turn off (or on) the saving of undo information. When
aligning very long overlaps it is advisable to turn off the undo saving.
When "Join/Quit" is pressed a dialogue box is displayed containing
the percentage mismatch of the overlap, and asking if the join should be made.
For joins above a certain
level of mismatch (20 percent by default) a second confirmation is required.
(Click for full size image)
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Last generated on 22 October 2002.
URL: http://www.mrc-lmb.cam.ac.uk/pubseq/manual/gap4_unix_67.html