This module analyses the traces at each base call to estimate a confidence
value for the called base. It does this by simply looking at the area
underneath the trace for the called base and dividing this by the highest area
under the trace for the three uncalled bases. This is a very simplistic
statistic which should ideally only be used for measuring the average
reliability of the entire sequence rather than any individual base. If another
program (eg Phred, or ATQA) is available then this should be used in
preference. From the 2002 release the eba values are
normalised to the phred scale (this was achieved by comaring the
original eba values and phred values for 4.6 million base calls of
Sanger Centre data).
There are no adjustable parameters for this module.
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Last generated on 22 October 2002.