Note the clash on AP which the io-lib uses for "Assembly Position" and PC which is used for "Position in Contig"
People to track: TP Template Prep person QP Sequencer Person, person who does sequencing reactions LP Loader Person AL Agar Loader person (when they run a gel to determine SI) AP Agar reaction Person (person who does the reactions to prepare the template to be run on a gel) Gel specific information GN Gel Name GL Gel Lane GP Gel Pourer person AG Agar Gel name (sizing gel) AF Agar Fate, no insert, no bands, what else? Name of library LB Library name, probably not critical to assembly even though one CN may have more than one library. But it is important to the cDNA project although I could put it in CN, since the cDNA project wouldn't have a CN otherwise. Processing information PC processing comment (a comment about PS) I think PS should just hold pass or fail and PC should hold additional information about why things passed. Trace information gotten from the ABI machine (from info field in SCF file): TS Trace Spacing DP Dye Primer HA signal strengtH A HG signal strengtH G HC signal strengtH C HT signal strengtH T (NOTE rs suggested these should go in a single record PP Primer Position (position at which primer peak was detected in trace) Stuff most likely specific to the cDNA project: MP Map Position TT Tissue Type of the library EI dbEst Id ER dbEst Remark OE Other Est's which are similar NI NCBI ID GB GenBank accession number SD Submission Date (when est was submitted) UD Update date (when it was last updated) CI citation associated with this cDNA